Method of Harvest Could Leave Embryos Undamaged
By Rick Weiss
Washington Post Staff Writer
Friday, January 11, 2008; A04
Scientists in Massachusetts said yesterday that they had created several colonies of human embryonic stem cells without harming the embryos from which they were derived, the latest in a series of advances that could speed development of stem-cell-based treatments for a variety of diseases.
In June, scientists in Japan and Wisconsin said they had made cells very similar to embryonic stem cells from adult skin cells, without involving embryos. But that technique so far requires the use of gene-altered viruses that contaminate the cells and limit their biomedical potential.
By contrast, the new work shows for the first time that healthy, normal embryonic stem cells can be cultivated directly from embryos without destroying them.
That means the work should be eligible for federal financing under President Bush's six-year-old policy of funding only stem cell research that does not harm embryos, said study leader Robert Lanza, chief scientific officer at Advanced Cell Technology in Worcester.
But that is not likely, said Story Landis, who heads the National Institutes of Health Stem Cell Task Force, which oversees grants for studies on the medically promising cells.
The embryos Lanza used, which were donated for research, appear not to have been damaged, Landis acknowledged. However, she said, "it is impossible to know definitively" that the embryos were not in some subtle way harmed by the experiment. And "no harm" is the basis of the Bush policy, she said.
Landis said the only way to prove that the technique does not harm embryos would be to transfer many of them to women's wombs and see whether the resulting babies were normal. But it would be unethical to do that experiment, she said, so the question cannot be answered.
That standard has Lanza fuming. By all scientifically recognized measures, he said, the embryos -- currently frozen in suspended animation because they were donated for research and not to make babies -- are normal, he said.
"I think the burden of proof lies with the NIH and the Bush administration to show that an embryo was harmed," Lanza said.
The new technique involves carefully removing a single cell from a newly formed eight-cell embryo and coaxing that cell to divide repeatedly until it forms a self-replenishing colony of embryonic stem cells.
Fertility doctors perform such "single cell biopsies" thousands of times every year to test the genetic health of embryos conceived by in vitro fertilization, with little or no apparent effect on the remaining seven cells' ability to develop into a normal baby. The idea is to check the removed cell for DNA defects and transfer to the woman's womb only embryos whose cells test normal.
Lanza's team first reported growing stem cells from individual embryo cells in 2006. But that work was criticized for not showing plainly that the plucked embryos could develop normally, relying instead on evidence from the nation's many fertility clinics that embryos can survive the process.
In the new experiments, he and his colleagues allowed their seven-cell embryos to continue growing in laboratory dishes for as many as five days -- the longest that embryos are typically cultured in fertility clinic labs before being transferred to a woman's uterus.
Of 43 embryos biopsied, 36 (or 84 percent) developed into healthy 5-day-old embryos, as determined by various measures used by the clinics, the team reported in yesterday's online edition of the journal Cell Stem Cell.
That's a survival rate as good as or better than that of fertility clinic embryos generally, whether they are biopsied or not, according to several published reports.